Request QuoteCatalog Number: xP382046PZFSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP382046PZFYeast1mgQuote
EP382046PZFE. coli1mgQuote
BP382046PZFBaculovirus200ugQuote
MP382046PZFMammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain MIT 9303)
UniProt IDA2C859
Gene NameleuA; Locus:P9303_09181
Protein Name2-isopropylmalate synthase
Region Expressed1-540
Expression Tag6xHis
Purity>90%
AA SequenceMAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASQGDFSA VQRIAQQVGGDEGPIICGLARASRADIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKS RAEVLAIVPEMVAYARSLVDDVEFSCEDAARSDPEFLYEVIEAAIAAGAGTINIPDTVGF TTPSEFGALIAGIDCHVPNMNEAILSVHGHNDLGLAVANFLEAVKSGARQFECTINGIGE RAGNAALEELVMALYVRRRYFNPFFGRESDSPTPLTAVRTEEITKTSRLVSNLTGMVVQP NKAIVGSNAFAHESGIHQDGVLKNRLTYEIVDARTVGLTDNRISLGKLSGRSAVRARLEE LGYDLTREDLDEAFARFKDLADRKRDITDRDLEAIVSEQVQQPEARFQLRLVQVSCGSSL RPTATVILAQEDGQEQTAAAVGTGPVDAVCRALNALAGEPNELIEFSVKSVTEGIDAMGE VTIRLRRDGQLFSGHSADTDVVVAAAQAFVNALNRLVAGCGRQSLHPQHDAVLADRRPGI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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