Request QuoteCatalog Number: xP381402PZHSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP381402PZHYeast1mgQuote
EP381402PZHE. coli1mgQuote
BP381402PZHBaculovirus200ugQuote
MP381402PZHMammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain NATL1A)
UniProt IDA2C3L7
Gene NameleuA; Locus:NATL1_15201
Protein Name2-isopropylmalate synthase
Region Expressed1-539
Expression Tag6xHis
Purity>90%
AA SequenceMAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASPGDFAA VQKIAENVGGEEGPIICGLSRASKPDIKACANAIAPAPKKRIHTFIATSDIHLEHKLRKS RKEVLDIVPDMVGYAKSFVDDVEFSCEDAARSDLDFLYEVIELAISSGANTINIPDTVGY ITPSEFGDLILNINENVPNINEAVLSVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE RAGNAALEELIMALHVRRSYFNPFFGRPPESPTPLTAVRTEEITKSSRLVSNLTGMVVQP NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLED LGYDLNREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPEALFQLKLVQVSCGTSL MPTATVTVVGEDGEEKTAVSLGTGPVDAVVRALDSLTEEPNELIEFSVKSVTEGIDALGE VTIRIRRDGNLFSGHSADTDVVVAAAQAYINALNRLVAAHGRKSIHPQHDLAKVDKKGI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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