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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP398194PYGYeast1mgQuote
EP398194PYGE. coli1mgQuote
BP398194PYGBaculovirus200ugQuote
MP398194PYGMammalian Cell200ugQuote

Protein Information

SpeciesPelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI)
UniProt IDA5D4W0
Gene NameleuA; Locus:PTH_0531
Protein Name2-isopropylmalate synthase
Region Expressed1-505
Expression Tag6xHis
Purity>90%
AA SequenceMSQRVYIFDTTLRDGEQSPGVSLNVGEKVQIARQLAKLGVDIIEAGFPITSPGDFKAVSE IARQVKGVTVAALARANFQDIDRAWEAVRHAEQPRIHTFIATSDIHLKYKLRMSREEVLD AAVAAVKRARAYTGDVEFSAEDASRSDLDFLCRVLAAAIEAGATVINIPDTVGYAVPEEW GKFINTIYHKVPGIEKVIVSVHCHNDLGMAVANSLAAVMNGARQVEGAINGIGERAGNAA IEEMVMALYTRKDQYNLYTNIKTEEIYRTSKLVSALTGMKVQPNKAVVGKNAFAHEAGIH QDGVLKERTTYEIMNPAMVGISKSNLVLGKHSGRHAFRHRLEEMGYNLSDEELNSAFERF KKLADKKMEITDEDLEAIIEEEMRLVPHTYTLEYLHISSGTTVVPTATVGLKRDGQLMEE AACGNGPVDAICKAIDKITGLNCTMTSWGINAVTAGKDALGDVSLKVTADGEKVYVGRGI STDVLEASAKAYVNAVNKLIWDSQK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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