Request QuoteCatalog Number: xP310569MOASize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP310569MOAYeast1mgQuote
EP310569MOAE. coli1mgQuote
BP310569MOABaculovirus200ugQuote
MP310569MOAMammalian Cell200ugQuote

Protein Information

SpeciesMicrocystis aeruginosa
UniProt IDP94907
Gene NameleuA
Protein Name2-isopropylmalate synthase
Region Expressed1-533
Expression Tag6xHis
Purity>90%
AA SequenceMNTSPDRVIIFDTTLRDGEQSPGAALNVDEKLTIARALARLGVDVIEAGFPHASPGDFEA VQKIAGSVGSEADSPIICGLARATQKDIKSAADALRPAAKPRIHTFLATSDIHLQYKLKK TRQEVLEIVPEMVAYAKSFLNDVEFSPEDAGRSDPEFLYQVLERAIAAGATTVNIPDTVG YTTPSEFGALIRGIKENVPNIDQAIISVHGHDDLGLAVANFLEAVKNGARQLECTINGIG ERAGNASLEELVMALHVRRSYFNPFLGRPAESTEPLTKINTKEIYRTSRLVSNLTGMIVQ PNKAIVGANAFAHESGIHQDGVLKHKLTYEIMDAESIGLTNNQIVLGKLSGRNAFRSRLQ ELGFELSETELNNAFIQFKEMADRKKEITDRDLEAIVNDEIDTVPDHFRLELVQVSCGNN ARPTATVTIRTPDGSELSDAAIGTGPVDALCKAIDRVVQIPNELISFSVREVTEGIDALG EVTIRLRYAGRTYSARAADTDIIVASARAYVSALNRLHVALQQKEKTPEMLQV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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