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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP601897Yeast1mgQuote
EP601897E. coli1mgQuote
BP601897Baculovirus200ugQuote
MP601897Mammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD) )
UniProt IDQ0BDB9
Gene NameleuA; Locus:Bamb_2298
Protein Name2-isopropylmalate synthase
Region Expressed1-514
Expression Tag6xHis
Purity>90%
AA SequenceMTDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKHLERMKVDVIEAGFAASSNGDFDAIHT IAGLVKDSTICSLARANDKDIQRAADALKPANSARIHTFIATSPLHMEKKLRMTPDQVFE QARLAVRFARKFTDNVEFSPEDGSRSDLDFLCRVLEAVIAEGATTINIADTVGYGVPELY GNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNT SLEEIVMAVKTRKDYFGLDVGLDTTQIVPTSKLVSQITGFVVQPNKAVVGANAFAHASGI HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVSLDSETELNAAFM RFKDLADRKSDIFDEDIIAIVSEESAFAQEQEHYKFVSLSQRSETGEQPQAKVVLALDGK EVTGEARGNGPVDATFNAIEGEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNG VGTDPDIVAASAKAYIAALNKLHSKDDKLNPQRS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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