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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP603714Yeast1mgQuote
EP603714E. coli1mgQuote
BP603714Baculovirus200ugQuote
MP603714Mammalian Cell200ugQuote

Protein Information

SpeciesHaemophilus somnus (strain 129Pt) (Histophilus somni (strain 129Pt) )
UniProt IDQ0I2G1
Gene NameleuA; Locus:HS_0392
Protein Name2-isopropylmalate synthase
Region Expressed1-517
Expression Tag6xHis
Purity>90%
AA SequenceMQNNIIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSAGDFESVQT IARHIKNSRVCALSRAMDKDIDIAADALKVAEAFRIHTFIATSALHVEAKLRRTFDDVVE MAIQAVKRARRYTDDVEFSCEDAGRTGVDNICRIVEAAIKAGATTVNIPDTVGFCLPTEY GNIINQVMNRVPNIDKAVISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTA LEEVVMAIKTRQDIFKGLDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGI HQDGMVKNKNTYEIMSPETIGLKKEKLNLTARSGRAAVKSHMDAMGYQQNDYDLDKLYAA FLKLADKKGQVFDYDLEALAFIDMQQGDEDRLSLDVITSQTISNLPASAFVQVELDGKKI SQVSNGGNGPVDAVYNAILAITDMDITMLHYNLTAKGEGAEALGQVDIVVEHKGRRFHGV GLATDIVESSARALIHAINAIYRANKVADLKSHKISQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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