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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP331984HTAYeast1mgQuote
EP331984HTAE. coli1mgQuote
BP331984HTABaculovirus200ugQuote
MP331984HTAMammalian Cell200ugQuote

Protein Information

SpeciesHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
UniProt IDP43861
Gene NameleuA; Locus:HI_0986
Protein Name2-isopropylmalate synthase
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMTDRVIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSQGDFESVQT IARHIKNARVAALSRAVDKDIDAAYEALKVAEAFRIHTFIASSALHVEAKLKRSFDDVVG MAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINAGATTVNIPDTVGFCLPNEY GNIIAQVRNCVPNIDKAVISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTS LEEVVMAMKVRQDFMGVDTHINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIH QDGMLKNKNTYEILSPETIGLKKEKLNLTARSGRAAVKGHMADMGYNEQDYDLDKLYDEF LKLADKKGQVFDYDLEALAFIDMQQGDEDRLVLDKLSAHSTKEYPATAFVQLKLDGEKLS TSSIGGNGPVDAVYNAILNLTGLEIKMSHYNLTAKGEGAEALGQVDIVVEHKGRKFHGVG LATDIVESSALALVHAINAIYRAHKVADIKNHKHH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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