Request QuoteCatalog Number: xP500926FFUSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP500926FFUYeast1mgQuote
EP500926FFUE. coli1mgQuote
BP500926FFUBaculovirus200ugQuote
MP500926FFUMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas fluorescens (strain SBW25)
UniProt IDC3K1K7
Gene NameleuA; Locus:PFLU_5050
Protein Name2-isopropylmalate synthase
Region Expressed1-559
Expression Tag6xHis
Purity>90%
AA SequenceMSMLKDPSSKYRAFPTIDIPDRTWPSKTITEAPIWCSSDLRDGNQSLIEPMDAVKKLRFW KTLVAVGVKEIEASFPAASQTDFDFVRTLIEDNHIPEDTTIQVLTQGREDLIARTFESLR GAKKAIVHLYNATSPSFRRIVFNQDKEGIKAIAVNAAKLFVKYAAQQPETQWTFEYSPET FSATELEFAKEVCDAVIEVWNPTPEHKMILNLPATVECATPNIYADQIEWFGRHINRRDS VIISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGLDPQ LDFSDIDGVRKVVEECNQIQVHPRHPYVGDLVHTAFSGSHQDAIRKGFTQQKDDALWEVP YLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYDISLPRRMQIEFSQVVQAETDRVG LEMTAPQIYALLQREYLQANTPYALVSHRLQEENGNSFVEVEVSGKGQGETNLHWKGKGN GALEALVAGLPIGVEIMDYNEHAIGAGTNAKAAAYIELRVNGERPVHGVGIDENITTASF KALFSALNRSLSQQEAKAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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