Request QuoteCatalog Number: xP500612LPXSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP500612LPXYeast1mgQuote
EP500612LPXE. coli1mgQuote
BP500612LPXBaculovirus200ugQuote
MP500612LPXMammalian Cell200ugQuote

Protein Information

SpeciesListeria monocytogenes serotype 4b (strain Clip81459)
UniProt IDC1KWT1
Gene NameleuA; Locus:Lm4b_01999
Protein Name2-isopropylmalate synthase
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVKAI AKAIKHCSVTGLARCVEGDIDRAEEALKDAVSPQIHIFLATSDVHMEYKLKMSRAEVLAS IKHHISYARQKFDVVQFSPEDATRSDRAFLIEAVQTAIDAGATVINIPDTVGYTNPTEFG QLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQ DGVLKNPDTYEIITPALVGVDKNSLPLGKLSGKHAFNTRMEEMGYTLTEQEQKDAFKRFK QLADAKKEVTEEDLHALILGQSSESADDFELKHLQVQYVTGGVQGAIVRIEERDGALVED AATGSGSIEAIYNTINRLMKQDIELTDYRIQAITAGQDAQAEVHVVIKNDKGAVFHGIGI DFDVLTASAKAYLQASGKSKTASKQADFEEVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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