Request QuoteCatalog Number: xP500071DPYSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP500071DPYYeast1mgQuote
EP500071DPYE. coli1mgQuote
BP500071DPYBaculovirus200ugQuote
MP500071DPYMammalian Cell200ugQuote

Protein Information

SpeciesAzotobacter vinelandii (strain DJ / ATCC BAA-1303)
UniProt IDC1DE40
Gene NameleuA; Locus:Avin_40120
Protein Name2-isopropylmalate synthase
Region Expressed1-557
Expression Tag6xHis
Purity>90%
AA SequenceMPMLKDPSQKYRPFAPIALRDRTWPDRVIDKAPLWLSTDLRDGNQSLIEPMDAAKKMRFF KTLVQVGLKEIEVGFPSASQTDFDFVRELIEGGHIPDDVTIQVLTQARDDLIERTFESLK GAKKAIVHYYNACAPSFRRIVFDQDKEGVKRIAVAAGRTIKRLAAAAPETRWGFEYSPEV FSSTESDFAVEVCNAVVEVFQPTPANRLILNLPATIECATPNHYADQIEWFCRHVDRRDS VIVSLHTHNDRGTGVAASELGLMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVDPG LDFSDIDAVRKVVEECNQLPVHPRHPYVGDLVHTAFSGSHQDAIRKGFAQQDPEGVWEVP YLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISLPRRMQIEFSQVVQKETDRLG LEMSAAQIHALLEAEYLRAETPYALKGHRLQEENGTCALDVEVFDKGESRHWRGIGKGPL EALVACLPVRVEIMDYHEHAIGAGSHARAAAYIELRLDGQRSLHGLGIDENLTTASIRAL FSALNRALGQQASIRAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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