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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP511934ESQYeast1mgQuote
EP511934ESQE. coli1mgQuote
BP511934ESQBaculovirus200ugQuote
MP511934ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6DEV8
Gene NameleuA; Locus:PC1_3607
Protein Name2-isopropylmalate synthase
Region Expressed1-532
Expression Tag6xHis
Purity>90%
AA SequenceMSEQVIIFDTTLRDGEQALQASLSVKEKLQIAFALERMGVDVMEVGFPVSSPGDFESVQT IARNIKNSRVCGLTRCVEKDIDVAAEALRVAEAFRIHTFIATSPMHIATKLRSTLDEVIE RAIYMIKRARNYTDDVEFSCEDAGRTPIPDLCRVVEAAINAGARTINIPDTVGYTMPHEF GNIISSLYQHVPNIDKAIISVHTHDDLGLAVGNAMAAVHAGARQVEGTLNGIGERAGNCS LEEVIMAIKTRHNILNVHTNINHQEIYRTSQLVSQICNMPIPANKAVVGANAFAHSSGIH QDGVLKNRENYEIMTPESIGLKEVQLNLTSRSGRAAVKHRMEEMGYQDSDYNLDDLYSAF LKLADKKGQVFDYDLEALAFINRQQEEPEFYHLDYFSVQSGSSVMATASVKLICGEETQS EAATGNGPVDAVYQAINRITGYQVSLVKYQLTAKGQGRDALGQVDIVADYQGRRFHGVGL ATDIVESSAQAMVNVLNNIKRAQQVEKEIQRLQQHNNQQQNDSKQQNSQETV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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