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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP498158AWKYeast1mgQuote
EP498158AWKE. coli1mgQuote
BP498158AWKBaculovirus200ugQuote
MP498158AWKMammalian Cell200ugQuote

Protein Information

SpeciesAcidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY) )
UniProt IDB9MA32
Gene NameleuA; Locus:Dtpsy_1948
Protein Name2-isopropylmalate synthase
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMTDKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERLRVDVIEAGFAASSNGDFESVQA IARAVKDSTVCSLARANDRDIARAAEALKEANRARIHTFIATSALHMEKKLRMTPEQVLE QARQAVRFARNLVEDIEFSPEDGYRSDPDFLCRVIETVIAEGATTINVPDTVGYAIPELY GNFIKNLRERIPNSDKAIWSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNC SLEEVVMAVKTRRDYFGLDVGIDTQHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGI HQDGVLKARDTYEIMRAEDVGWSANKIVLGKLSGRNAFKQRLQELGVQLESEGEINVAFA KFKELADRKSEIFDEDILALVSDESVTSEKEQFGFVSLAQHSETGERPEATIVFTVDGKE VRSSAEGNGPVDASMKAIEAHVRSGAEMVLYSVNAISGSTESQGEVTVRLQSAGRVVNGV GADPDIVVASAKAYLSALNKLQSKAERVAAQG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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