Request QuoteCatalog Number: xP507705BQKSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP507705BQKYeast1mgQuote
EP507705BQKE. coli1mgQuote
BP507705BQKBaculovirus200ugQuote
MP507705BQKMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus (strain 03BB102)
UniProt IDC1EMA9
Gene NameleuA; Locus:BCA_1455
Protein Name2-isopropylmalate synthase
Region Expressed1-506
Expression Tag6xHis
Purity>90%
AA SequenceMKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRI ANTIQNATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS IYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRAGANVINIPDTVGYTNPEEYY SLFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQ DGVLKEVTTYEIIEPALVGESQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFK KLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVFED AATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA QDVLEASARAYVHAAGKLKSFIQLVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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