Request QuoteCatalog Number: xP510544DQMSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP510544DQMYeast1mgQuote
EP510544DQME. coli1mgQuote
BP510544DQMBaculovirus200ugQuote
MP510544DQMMammalian Cell200ugQuote

Protein Information

SpeciesCatenulispora acidiphila (strain DSM 44928 / NRRL B-24433 / NBRC 102108 / JCM 14897)
UniProt IDC7QJN7
Gene NameleuA; Locus:Caci_2342
Protein Name2-isopropylmalate synthase
Region Expressed1-573
Expression Tag6xHis
Purity>90%
AA SequenceMPSHRYRSVFDRVEVPLTDRTWPDQRFSAAPLWVPVDLRDGNQALAEPMDTARKTKMFDL FLRMGYKEIEVAYPSASQTDFDFVRSLAVRDDIPEDVTISVFTPARPELIERTFESVEGL PNVLVHLYTATAPAWRDLVLRGSRAEVLALIEDAAGLVAKLADQRLGTKHGPGAGRMRFE FSPEVFNLTEPDYVIEVCDRVTEIWDACADRPVVHNLPATVEVATPNVYADQIEYMSRSL ARREHVILSVHPHNDRGTGVACAELAVLAGAQRVEGCLFGNGERTGNVDLVTLALNLHAQ GVDPMIDLSDIDAVKAVVEECNRIPVHVRHPYGGDLVYTAFSGTHQDAISKGMAHHAARA AELGVPASQAPWDVPYLPIDPADVGRSYQGVIRVNSQSGKGGIAYLLRTHYGVDLPRDFQ PGFSKVVQRATDEHGREMTPQDLWELFRTTYVVPGEDGAWKLTDTAFQEIEGGGHRVEAT AVRADGTVIPVAGIGNGPLSALTVALAKAGIGFEVVDYTENAVYSEHAVSEGADAVAVCY IRARVEGREVWGVGMDTSVLTAMVRAAVSAVNR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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