Request QuoteCatalog Number: xP510219GFJSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP510219GFJYeast1mgQuote
EP510219GFJE. coli1mgQuote
BP510219GFJBaculovirus200ugQuote
MP510219GFJMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus sp. (strain WCH70)
UniProt IDC5D5M0
Gene NameleuA; Locus:GWCH70_2593
Protein Name2-isopropylmalate synthase
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMRKINIFDTTLRDGEQSAGINLNLQEKLEIARQLERLGVDIIEAGFPASSKGDFQAVKQI AETIKTCSVTGLARSVQSDIDAAWEALKGGAEPRLHLFIATSPIHMKYKLQMTPEQVIET AVESVKYAKRYFPIVQWSAEDACRSELPFLAKIITEVIKAGATVINIPDTVGYITPKEYG NIFTFLSNNVPNIEKVSLSAHCHDDLGMAVANSLAAIEHGATQIEGTINGIGERAGNAAL EEIAVALYIRKDYYQAETRLNLQEIKRTSNLVSKLTGVVIPPNKAVIGKNAFAHESGIHQ DGVLKEKTTYEIISPELVGVQSNSMVLGKHSGRHALRNRVEELGYTLSDEEVNKLFVRFK ELADKKKDITDDDLVALIFEEKFDHFKDFYQLSSLQVQYGTNQIPTAVVVLKDGQGNEIQ EAATGAGSVEALYNTLERCFKTSVTLLDYRIESVSGGRDALAQVFVKVRVNDIETSGRGT AQDVLEASAKAYINAVNRVFMIETMRAENQKVAMQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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