Request QuoteCatalog Number: xP501587BQGSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP501587BQGYeast1mgQuote
EP501587BQGE. coli1mgQuote
BP501587BQGBaculovirus200ugQuote
MP501587BQGMammalian Cell200ugQuote

Protein Information

SpeciesBacillus anthracis (strain CDC 684 / NRRL 3495)
UniProt IDC3L9Q7
Gene NameleuA; Locus:BAMEG_3175
Protein Name2-isopropylmalate synthase
Region Expressed1-506
Expression Tag6xHis
Purity>90%
AA SequenceMKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRI ANTIQNATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS IYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRAGANVINIPDTVGYTNPEEYY ALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVSKNKAIVGANAFAHESGIHQ DGVLKEVTTYEIIEPALVGESQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFK KLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVFED AATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA QDVLEASARAYVHAAGKLKSFIQLVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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