Request QuoteCatalog Number: xP521698BXHSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP521698BXHYeast1mgQuote
EP521698BXHE. coli1mgQuote
BP521698BXHBaculovirus200ugQuote
MP521698BXHMammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Thelaxes suberi
UniProt IDO31287
Gene NameleuA
Protein Name2-isopropylmalate synthase
Region Expressed1-375
Expression Tag6xHis
Purity>90%
AA SequenceGRTSIDNLCRIVESAIKAGATTINIPDTVGYTIPSQFKTIIKNLFNKVPNINNAIISAHC HDDLGLAVGNAIASIEAGVRQIEGTISGIGERAGNTALEEVILTIKLKEELLNVFTNIKH NELYRTSQLISQLCNTPIPANKSIIGSNAFAHSSGIHQDGILKNRANYEIIDPKTIGVKA VQLNLTSRSGRAAVKHHMHEMGYQDDDYDLNQLYVNFLKLADKKGQVFDYDLEALAFISN QEDKDQEHFQLKYFSIHSGSTGVSTASVKLLCGNKILNEIITTRNGPINSIYQALNNIAG LPIVLEKFHLVGKGEGRDALGQVDIVVKYNNRKFHGIGLATDIIESSIKAMLNVLNNIWL SNKIKSKIKNIKNKK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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