Request QuoteCatalog Number: xP523233FOBSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP523233FOBYeast1mgQuote
EP523233FOBE. coli1mgQuote
BP523233FOBBaculovirus200ugQuote
MP523233FOBMammalian Cell200ugQuote

Protein Information

SpeciesStreptomyces coelicolor (strain ATCC BAA-471 / A3 (2) / M145)
UniProt IDO31046
Gene NameleuA; Locus:SCO2528; ORFs:SCC117.01c, SCC121.31c
Protein Name2-isopropylmalate synthase
Region Expressed1-573
Expression Tag6xHis
Purity>90%
AA SequenceMANRQQPSPMPTAKYRGYDQVDIADRTWPNQRITTAPRWLSTDLRDGNQALIDPMSPVRK RAMFDLLVKMGYKEIEVGFPASGQTDFDFVRSIIEEPGAIPDDVTISVLTQAREDLIERT VESLKGARRATVHLYNATAPVFRRVVFRGSRDDIKQIAVDGTRLVMEYAEKLLGPETEFG YQYSPEIFTDTELDFALEVCEAVMDTYQPGPGREIILNLPATVERSTPSTHADRFEWMGR NLSRREHVCLSVHPHNDRGTAVAAAELALMAGADRIEGCLFGQGERTGNVDLVTLGMNLF SQGVDPQIDFSDIDEIRRTWEYCNQMEVHPRHPYVGDLVYTSFSGSHQDAIKKGFDAMEA DAAARGVTVDDIEWAVPYLPIDPKDVGRSYEAVIRVNSQSGKGGIAYVLKNDHSLDLPRR MQIEFSKLIQAKTDAEGGEITPTAIWDVFQDEYLPNPDNPWGRIQVANGQTTTDRDGVDT LTVDATVDGAETTLVGSGNGPISAFFHALQGVGIDVRLLDYQEHTMSEGASAQAASYIEC AIGDKVLWGIGIDANTTRASLKAVVSAVNRATR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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