Request QuoteCatalog Number: xP503351BQFSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP503351BQFYeast1mgQuote
EP503351BQFE. coli1mgQuote
BP503351BQFBaculovirus200ugQuote
MP503351BQFMammalian Cell200ugQuote

Protein Information

SpeciesBacillus anthracis (strain A0248)
UniProt IDC3P4Z6
Gene NameleuA; Locus:BAA_1487
Protein Name2-isopropylmalate synthase
Region Expressed1-506
Expression Tag6xHis
Purity>90%
AA SequenceMKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRI ANTIQNATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS IYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRAGANVINIPDTVGYTNPEEYY ALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVSKNKAIVGANAFAHESGIHQ DGVLKEVTTYEIIEPALVGESQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFK KLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVFED AATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA QDVLEASARAYVHAAGKLKSFIQLVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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