Request QuoteCatalog Number: xP497635BQQSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP497635BQQYeast1mgQuote
EP497635BQQE. coli1mgQuote
BP497635BQQBaculovirus200ugQuote
MP497635BQQMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus (strain Q1)
UniProt IDB9IUZ0
Gene NameleuA; Locus:BCQ_1473
Protein Name2-isopropylmalate synthase
Region Expressed1-506
Expression Tag6xHis
Purity>90%
AA SequenceMKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRI ANTIQNATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS IHRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRAGANVINIPDTVGYTNPEEYY SLFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL EEVAVALHIRKDFYQAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQ DGVLKEVTTYEIIEPELVGESQNLFVLGKHSGRHAFTEKMKELGYEFTTEERDAVFEAFK KLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVFED AATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGTHQVSGFGVA QDVLEASARAYVHAAGKLKSFIQLVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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