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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP494686DUQYeast1mgQuote
EP494686DUQE. coli1mgQuote
BP494686DUQBaculovirus200ugQuote
MP494686DUQMammalian Cell200ugQuote

Protein Information

SpeciesClostridium kluyveri (strain NBRC 12016)
UniProt IDB9E371
Gene NameleuA; Locus:CKR_1895
Protein Name2-isopropylmalate synthase
Region Expressed1-514
Expression Tag6xHis
Purity>90%
AA SequenceMTKKIYIFDTTLRDGEQTPKVSLNINDKITIAKQLQKLSVDVIEAGFPKASHGDFEAVKA IAESIQGPVIVGLARASKEDIDCAWEALKGSLKPRIHIFLATSDIHMEHKLKMKPEEVLK RAADMVRYAKGLCPSIEFSPEDATRTRPEFLYKVLEAVIEAGADVVNIPDTVGYTTPVEY GAFIRGIKENVKNIEDAIISVHCHNDLGLAVANSLAAIESGAEQVECAINGLGERAGNAA LEEIVMAISTRADSFNCHTDIVTEEITKTSSIVSHVTGMQVQGNKAIVGANAFAHESGIH QHGVLNCRETYEIMTPESVGLKKNFIVLGKHSGRHAFVEHLHEMGYKDLSVEKTDEIFKK FKELADKKKHISDEDIESLVKNEIFHVPEVFKLKYYQVFTGNTVVSTSTVEIECNGKKLS EASCGDGPVDATFKAIEKATGIDVTLNDYFIKAVGSGKDAMGEVTVRIEKEGKIFSAKGI STDIVEASGIAFINAVNKLYYETYSKDLQKISAN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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