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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP522211DUSYeast1mgQuote
EP522211DUSE. coli1mgQuote
BP522211DUSBaculovirus200ugQuote
MP522211DUSMammalian Cell200ugQuote

Protein Information

SpeciesClostridium sticklandii (strain ATCC 12662 / DSM 519 / JCM 1433 / NCIB 10654)
UniProt IDE3PVH7
Gene NameleuA; Locus:CLOST_0414
Protein Name2-isopropylmalate synthase
Region Expressed1-567
Expression Tag6xHis
Purity>90%
AA SequenceMNYKKYKGYETIVLEDRNWPSRKIEKAPQWCSVDLRDGNQALITPMGLKQKLRFFEYLVK MGFKTIEIGFPAASDTEYEFTRTLIEEGYIPEDVTIQVLTQSRIQIINKTFEALKGTKNA VVHLYNSTSTLQREVVFRNSKEETIELAVFGARAIKELALKNPETNFTFEYSPESFTGTE MDFAAEICNSVIDVWKPTKDKKVIINLPSTVEMATPNTYADQIEYMCRNLKSRENIIVSL HAHNDRGTGVAATELGLMAGADRVEGTLFGNGERTGNADILNLGMNLYTQGINPELDFSD INSMIEIYEESTAMNVHPRHPYAGDLVFTAFSGSHQDAIKKGMARMNKQAQYWEVPYLPL DPKDIGKSYEPIIRINSQSGKGGVSFILEQNYGLYIPKDFQKDAGRVITAYSDKMQTEIG AEEIYEVFLNEYVDIRTPLQLNYHKTQSVDSDWDLVFIEAEIEYNMQKHYVQAEGNGVVS AFCNSLKEIIGMDIDIVDYRGHSMEYGTKAKAISYIELKNETGERFYGAGTSSSVGKSSL RAVVSAINKMILAQQTKDMDTQEEDIA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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