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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP517173FKJYeast1mgQuote
EP517173FKJE. coli1mgQuote
BP517173FKJBaculovirus200ugQuote
MP517173FKJMammalian Cell200ugQuote

Protein Information

SpeciesSpirochaeta smaragdinae (strain DSM 11293 / JCM 15392 / SEBR 4228)
UniProt IDE1R5X1
Gene NameleuA; Locus:Spirs_1609
Protein Name2-isopropylmalate synthase
Region Expressed1-505
Expression Tag6xHis
Purity>90%
AA SequenceMPRRIKIFDTTLRDGEQSPGASMSLEQKIKMASALERLGVDRIEAGFPVSSPVQFEAVQR VSATVKKAEVVGLARCVQRDIDAAYDALRDAAHPMLHIFIATSPLHREYKLKKSKEEILD TVRECLNYGGKGFSRMEFSPEDASRTEPDYLVEVVKTAIECGATSINIPDTVGYAVPKEF GQLISFLVEQVPQFSDGSVDLSVHCHNDLGLALANSLAAVRSGASQVEVTLNGIGERAGN CPMEELIMSLDVRKDMFDVETGIHTEYLYPTGKLLQSITGLLIPRNKPIFGDNVFVHESG IHQHGVLNKRETYEIMKPERIGRSSETIIMGRHSGKHALEDKLSQYNIKLTRQQFEDVFA RFTAIADKKKEVYDEDLFSIVGTVLGGVVKGFSLLYFHTFTGNSLIPGATVKVRSEAGEK VASATGDGPVDAVFNAIDECVGINARLKEYIIHAIGSGKDAQGEVKLEVEIEGATYGGKA SSTDIIEASAMAYLNAVNRFELRKR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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