Request QuoteCatalog Number: xP494791FLKSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP494791FLKYeast1mgQuote
EP494791FLKE. coli1mgQuote
BP494791FLKBaculovirus200ugQuote
MP494791FLKMammalian Cell200ugQuote

Protein Information

SpeciesStaphylococcus carnosus (strain TM300)
UniProt IDB9DMJ6
Gene NameleuA; Locus:Sca_1563
Protein Name2-isopropylmalate synthase
Region Expressed1-510
Expression Tag6xHis
Purity>90%
AA SequenceMSHIQVFDTTLRDGEQTPGVSFSFDERLTIAKQLEKWGVDVIEAGFPASSQGSFDSVKAI SETLTKTAVVGLARCKKSDIDAVYEATKDAKYPQLHVFIASSPIHLEHKLKMTQEELLEN ITENVSYGKSLFDVVQFSPEDATRTDLDFLVKCVQTAVDAGASIINIPDTVGFSYPSEFG NIFKVLIENVKSDHEVTYSAHCHDDLGLAVANSMAAIENGATRIEGAVNGIGERAGNTAL EEVALGLYVRRDHYGIETNLKLDETKATSDLVASFAGIRVPRNKAIVGQNAFSHESGIHQ DGFLKHPETYEIMTPQLVGIKSSELPLGKLSGKHAFAEKLKQLGYDITAEKQVELFKQFK SIADKKKNVSDHDVHALVQGHSHETNAAYQVETLQLQFVSEGVQSAVVVLKDHEGKEYPS AAIGTGSIVAVYNAVDQIFKAPSELLNYQITSVTEGSDAQAQVNVEIRIDGKTYYGIGVD HDVLLASCKAYVEANSNHLADTARKDGVVS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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