Request QuoteCatalog Number: xP469328AZMSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP469328AZMYeast1mgQuote
EP469328AZME. coli1mgQuote
BP469328AZMBaculovirus200ugQuote
MP469328AZMMammalian Cell200ugQuote

Protein Information

SpeciesAliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238) )
UniProt IDB6ELK0
Gene NameleuA; Locus:VSAL_I0377
Protein Name
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMSDQVIIFDTTLRDGEQALSASLTVKEKLQIAFALERLGVDVIEAGFPISSPGDFESVRT IAKHIKNSRICALSRAVTKDIDVAADALKVAEAFRIHTFISTSTVHVQDKLRRSYDDVVE MGVKAVQRARKYTDDVEFSCEDAGRTPIDNLCRMVEAAINAGANTINIPDTVGYTIPGEF GGIIQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAVQAGARQVEGTINGIGERAGNCS LEEIAMILQTRSEYLGVHTSLKNDEIHRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIH QDGMLKNKNTYEIMTPESIGLKNKALNLTSRSGRAAVKSHMDSMGYKAEEYNLDTLYADF VKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQSGSVMATTSIKLLCGDEEKCE AAVGNGPVDALYQCIYKLTGYEIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGLA TDIIEASGQALLHVINSIHRADQIAEIKQNKITTV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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