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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP479840SWOYeast1mgQuote
EP479840SWOE. coli1mgQuote
BP479840SWOBaculovirus200ugQuote
MP479840SWOMammalian Cell200ugQuote

Protein Information

SpeciesSalmonella enteritidis PT4 (strain P125109)
UniProt IDB5R2K9
Gene NameleuA; Locus:SEN0114
Protein Name2-isopropylmalate synthase
Region Expressed1-523
Expression Tag6xHis
Purity>90%
AA SequenceMSQQVIIFDTTLRDGEQALQASLSAKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQT IARTIKNSRVCALARCVEKDIDVAAQALKVADAFRIHTFIATSPMHIATKLRSTLDEVIE RAVYMVKRARNYTDDVEFSCEDAGRTPVDDLARVVEAAINAGARTINIPDTVGYTMPFEF AGIISGLYERVPNIDKAIISVHTHDDLGIAVGNSLAAVHAGARQVEGAMNGIGERAGNCA LEEVIMAIKVRKDIMNVHTNINHHEIWRTSQTVSQICNMPIPANKAIVGSGAFAHSSGIH QDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMEEMGYKDTDYNMDHLYDAF LKLADKKGQVFDYDLEALAFINKQQEEPEHFRLDYFSVQSGSSDIATASVKLACGEEIKA EAANGNGPVDAIYQAINRITGYDVELVKYDLNAKGQGKDALGQVDIVVNHHGRRFHGVGL ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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