Request QuoteCatalog Number: xP467221OEDSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP467221OEDYeast1mgQuote
EP467221OEDE. coli1mgQuote
BP467221OEDBaculovirus200ugQuote
MP467221OEDMammalian Cell200ugQuote

Protein Information

SpeciesOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)
UniProt IDB6JB87
Gene NameleuA; Locus:OCAR_5296, OCA5_c26790
Protein Name
Region Expressed1-519
Expression Tag6xHis
Purity>90%
AA SequenceMTSPSTSDKDRVVIFDTTLRDGEQCPGATMTHEEKLEVAEMLDTMGVDIIEAGFPIASVG DFEAVAEIAKRANNAVIAGLARAIPADIVRAGEAVRHAKRGRIHTFVSTSPIHLAHQMRK SEEDVLGIITATVAQARNLVDDVEWSAMDSTRTPIDYLCRCVETAIAAGATTINLPDTVG YAVPEEYRRMFKVIRERVPNADKAVFSVHCHDDLGLAVANSLAGVEGGARQIECTINGIG ERAGNAALEEVVMAIKTRGDVMPYWCNVESTTLTRASKVVSAATSFPVQYNKAIVGRNAF AHESGIHQDGVLKNAQTYEIMTPESVGVKQTSLVMGKHSGRHAFQHKLRELGYELADNQL QDAFVRFKALADRKKDIYDEDIEALVDQELAQTHDRLRLVSLTVIAGTHGPQRATMKVEV DGKVRIDEAEGNGPVDAVFNAVKTLVPHEAKLELYQVHAVTAGTDAQAEVSVRLSQDGRS VTARSSDPDTLVASAKAYLSALNKIISRQQREAPVAAAS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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