Request QuoteCatalog Number: xP462963MSVSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP462963MSVYeast1mgQuote
EP462963MSVE. coli1mgQuote
BP462963MSVBaculovirus200ugQuote
MP462963MSVMammalian Cell200ugQuote

Protein Information

SpeciesMethylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4) )
UniProt IDB3DX88
Gene NameleuA; Locus:Minf_1743
Protein Name
Region Expressed1-506
Expression Tag6xHis
Purity>90%
AA SequenceMEMTANRLIVFDTTLRDGEQCPGASMTSRQKLEVAKQLARLGVDVIEAGFPVISQGDFES VSEIAAQVKGPKICGLARCLPKDIEAAAEALKAAADAARIHVFLATSQIHRRFKLAKDEE EIVRIAVEGVRLAKRYVQDVEFSAEDASRTEPEFLAKIIQKVIEAGATTVNIPDTVGYAV PEEFASLIRYLFDHVQDIDKVVVSVHCHNDLGLAVSNSLAAIKAGARQVEGTINGIGERA GNAALEEIIMALHTRPDAFGKIETGIQLKEILRTSRLVSRMSGLAVQRNKAVVGENAFAH AAGIHQDGILKKRETYEIIDPKIIGWEQSELPLTKHSGRAALENRLKILGFELEKEEIDN IFSQFKQIGDKKKFIYDDDLVSLVEGQISRIKETYELEYVAVTVCSGGIPMATIKLRHGE EVLVDASTGDGAVDAAMKAVDRITGQHGHLVEYEVKSVTEGKDAIGEVTVKVNFDSKKLV TAKAASTDVIEASIKAYLNAVNKALL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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