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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP452040EXMYeast1mgQuote
EP452040EXME. coli1mgQuote
BP452040EXMBaculovirus200ugQuote
MP452040EXMMammalian Cell200ugQuote

Protein Information

SpeciesPolynucleobacter necessarius (strain STIR1)
UniProt IDB1XUJ3
Gene NameleuA; Locus:Pnec_0815
Protein Name
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMSDKVIIFDTTLRDGEQSPGASMTKDEKVRIARQLERLKVDVIEAGFAASSEGDFQAISA VAAAVKDSIVCSLARANDKDITRAADALQATNAKRIHAFLATSPLHMAVKLRMSPEEVLE QAKRSIRFACNLASDIEFSAEDGYRSEMDFLCRVVEAVINEGASTINIPDTVGYATPELY GEFIKTLRTRVPNSDKAVWSVHCHNDLGMAVANSLAGVKIGGARQIECTINGLGERAGNT ALEEIVMSLRTRKDYFDMVCGIDATQIVPASKLVSQITGFIVQPNKAVVGANAFAHTSGI HQDGILKNRDTYEIMRAEDVGWSANKIVLGKLSGRNAFKQRLQELGITVEAEADLNVAFT RFKALADQKSEIFDEDIIAIMSDSAAAEEGEHYKFISLSQHSETGERPKSRVTFRMGDRE ISSEAEGNGPVDASLNAIEGIAKSGAEQLLYSVNAITSGTQSQGEVTVRLAKGGRIVNGV GTDPDIIAASAKAYLSALNKLHDPSQAKLNAQMTP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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