Request QuoteCatalog Number: xP492212HUESize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP492212HUEYeast1mgQuote
EP492212HUEE. coli1mgQuote
BP492212HUEBaculovirus200ugQuote
MP492212HUEMammalian Cell200ugQuote

Protein Information

SpeciesHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
UniProt IDB8CX21
Gene NameleuA; Locus:Hore_10860
Protein Name2-isopropylmalate synthase
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMGEVKIFDTTLRDGEQSPGVSLIPEEKLAIAKQLARMKVDVIEAGFPISSPGDFEAVQII SENIRDVEVAALARSRKKDIDRAWEALRNGGDPRIHVFIATSPIHMKYKLKLSEEQVIEK AVEAVKYASKYTSNIEFSAEDASRSQPVFLYRLFERVINAGAKVINIPDTVGYAIPEEFG KLIRDIKENVSNIDKVDISVHCHNDLGLAVANSLAAVENGANQIEVAVNGIGERAGNTAL EEIIMALYTRKDFYNIGINQDTTQIARLSKLVSNLTGMTIQPNKAIVGANAFAHESGIHQ DGVIKERTTYEIMDARTIGLKDNKLVLGKHSGRHAFREFIQKLGYDIDDETFEEIFIEFK KLADKKKNITHVEIEALIDNHYHTFDKVYELDYLSVNTGNKVLPTATIKLKKENNIIEKA ACSGDGPVDAIFQAINEIVGIDDIKLISYHINAVTEGKDALGEVIVKTKIEDNTYTGHSA MTDITEASALAYLETINKFLTSEQTRQTTSAQEGI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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