Request QuoteCatalog Number: xP490837VFHSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP490837VFHYeast1mgQuote
EP490837VFHE. coli1mgQuote
BP490837VFHBaculovirus200ugQuote
MP490837VFHMammalian Cell200ugQuote

Protein Information

SpeciesVibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32) )
UniProt IDB7VIF2
Gene NameleuA; Locus:VS_0380
Protein Name2-isopropylmalate synthase
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMNDQVIIFDTTLRDGEQALAASLTVKEKLQIAYALERLGVDVIEAGFPISSPGDFESVQT IAKHIKDSRICALSRAVAKDIDAAAEALKVADQFRIHTFISTSTVHVQDKLRRSYDDVVE MAVKAVKHARNYTDDVEFSCEDAGRTPIDNLCRMVEAAINAGAKTINIPDTVGYTVPNEF GGIIKTLFDRVPNIDQAIISVHCHDDLGMSVANSIAAVQAGARQIEGTINGIGERAGNCS LEEIAMIIKTRQELLGVHTGLDHKEIHRTSKLVSQLCHMPIQDNKAIVGANAFSHSSGIH QDGMLKNKNTYEIMTPESIGLKNKALNLTSRSGRAAVKSHMDAMGYKDNEYNLDSLYEDF LKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQSGSVMSTTSIKLQCGDEEKCE AAVGNGPVDALYQCIYRLTGYEIALDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGVS TDIVEASGQALLHVINSIQRADTIAEMKQQKFATV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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