Request QuoteCatalog Number: xP474373FLYSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP474373FLYYeast1mgQuote
EP474373FLYE. coli1mgQuote
BP474373FLYBaculovirus200ugQuote
MP474373FLYMammalian Cell200ugQuote

Protein Information

SpeciesStenotrophomonas maltophilia (strain R551-3)
UniProt IDB4SI67
Gene NameleuA; Locus:Smal_3332
Protein Name
Region Expressed1-528
Expression Tag6xHis
Purity>90%
AA SequenceMTTIERITTPRIRIFDTTLRDGEQSPGCSMSPPQKLVMARALDELGVDIIETGFPASSQS DRDAMALIGRELRRPSLSLAVLSRCLQADIETSARALEAAANPRLHVFLSTSPLHREHKL RMTREQVLESVRKHVSLARSYIDDVEFSAEDATRTELDYLIEVSRAAISAGATTINLPDT VGFTTPEEIREMFQQVIAGVADVPNAANVIFSAHCHNDLGLAVANSLAAIEGGARQVECT VNGIGERAGNCSLEEIAMVLKVRQAFYEQDSSINTPRIVGTSQLLQRLVGMPVQRNKAIV GANAFAHESGIHQHGMLRHRGTYEIMRPEDVGWEDSQMVLGRHSGRAAVEARLRALGFWL EEDELKLVFEQFKGLCEQQRVVTDADLQTLMQGGSNAQGYRLASMTISDVGSRANALVEL SDPDGNRVAETAQGDGPVDALFGALSAATGVQLMLDSYHVHSVGIGADARGEANLSVRHE GVEYDGTGTSKDIIEASALAWLDVANRLLRQRQANAASTTEAPAPATA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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