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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP454121MTCYeast1mgQuote
EP454121MTCE. coli1mgQuote
BP454121MTCBaculovirus200ugQuote
MP454121MTCMammalian Cell200ugQuote

Protein Information

SpeciesMethylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)
UniProt IDB1ZDN3
Gene NameleuA; Locus:Mpop_2796
Protein Name
Region Expressed1-520
Expression Tag6xHis
Purity>90%
AA SequenceMANTTESAKDRVVIFDTTLRDGEQCPGATMTLDEKLAVAELLDAMGVDIIEAGFPIASNG DFEAVSEIARRTKRATIAGLARAIPADIARAGEAVRHAKAGRIHTFVSTSAIHLAHQMRK TQDEVIEIILKTVTQARDLVEDVEWSAMDATRTDIDYLCRCVEAAIRSGATTINLPDTVG YATPQEYGAMFRQVRERVPNSDKAIFSVHCHNDLGLAVANSLAGLEGGARQIECTVNGIG ERAGNAALEEIVMAIRTRADVMPYDTGIDTTMLTRASKLVSHAANFPVQYNKAIVGRNAF AHESGIHQDGMLKHSETYEIMTPASVGLSKTSLVMGKHSGRAAFKSKLAELGISLSDNQF QDVFERFKDLADRKKHVYDEDIEALVDEKLATAHDRIKLLSLSVIAGTRGPQRATMKIEM DGRTFTEEADGNGPVDAVFNAIHEIVPHDAVLELYQVHAVTEGTDAQAEVSVRLKAGERS VTARGADPDTLVASAKAYLSALNKLSAASVRLHAQHAAVV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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