Request QuoteCatalog Number: xP478508BTBSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP478508BTBYeast1mgQuote
EP478508BTBE. coli1mgQuote
BP478508BTBBaculovirus200ugQuote
MP478508BTBMammalian Cell200ugQuote

Protein Information

SpeciesBifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
UniProt IDB7GT76
Gene NameleuA; Locus:Blon_0170, BLIJ_0173
Protein Name2-isopropylmalate synthase
Region Expressed1-638
Expression Tag6xHis
Purity>90%
AA SequenceMGQDQSSVFDLAAVAAASNGGNNDPLLPPARFIGDPQKPSRMPYNKYASYSEQIPFDYPE RTWPGKRLQRAPRWCSVDLRDGNQALVNPMDSERKLRFWNLLVSMGFKEIEVGFPSASET DFDFIRMLIERELIPDDVTIVVLTQCREHLIRRTYEALKGAKRAIVHFYNSVSVLQREVV FRKNKEEIKKLATDAAELCKDLENEAKGIDLYYEYSPESFTGTEPEYAVEVCNAVIGVIK PTPEHPMIINLPATVEMTTPNVFADEVEYVSTHLDDRDSVVLSLHPHNDEGMGVAATELA VLAGADRVEGCLLGNGERTGNVDLVTLGLNWLTQGIDPQLDLSNVPEIRKTVEYCNQIKI SERHPYAGNFVFTAFSGSHQDAIKKGLEARQVAAERAGADLDSFVWLVPYLPIDPKDIGR TYEAIIRVNSQSGKGGMAYLLKTNHNLDLPKRLQIEFDKIVQNYADTTKKEVKDGDIWRL FKDEYLPVEQSGMTAAGVVVGDTHDASLEPWGRLKLLKVAVSSGEDGSDTVLKARLLDRG VNVGDDEPVEREASGIGNGPIAAFLNAISNFGVEASIMDYVEHTMSVGTDAMAASYVECQ IGEADDAQIVWGVGIDSSITTSALKAIISAINRSQRQR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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