Request QuoteCatalog Number: xP493034SYSSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP493034SYSYeast1mgQuote
EP493034SYSE. coli1mgQuote
BP493034SYSBaculovirus200ugQuote
MP493034SYSMammalian Cell200ugQuote

Protein Information

SpeciesShewanella baltica (strain OS223)
UniProt IDB8E4K4
Gene NameleuA; Locus:Sbal223_0411
Protein Name2-isopropylmalate synthase
Region Expressed1-522
Expression Tag6xHis
Purity>90%
AA SequenceMSNRVIIFDTTLRDGEQALAASLSVKEKLQIAMALERLGVDVMEVGFPVSSPGDFESVQT IARTIKNSRVCALSRALEKDIDAAAQALSVADQFRIHTFISTSTIHVESKLKRSFDQVLE MAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAILVGARTINIPDTVGYTVPSEF GTIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTINGIGERAGNCS LEEIAMILATRKGMLGLETGINAKEIHRTSNLVSQLCNMPVQANKAIVGANAFTHSSGIH QDGMLKAQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMEEMGYSEHDYNMDALYEEF LKLADKKGQVFDYDLEALAFMEAQAEEDNHYQLQQLVVQSDSTEGVATATVRIEVGGEIK TEAATGNGPVDAAYNAIARATDRRIDIISYKLGAKGVGQNALGQVDITAVYHEQNFHGVG LATDVVEASARALVHVMNLTCRADKVADYKQSMQKNRELGGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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