Request QuoteCatalog Number: xP489742LPWSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP489742LPWYeast1mgQuote
EP489742LPWE. coli1mgQuote
BP489742LPWBaculovirus200ugQuote
MP489742LPWMammalian Cell200ugQuote

Protein Information

SpeciesListeria monocytogenes serotype 4a (strain HCC23)
UniProt IDB8DBU4
Gene NameleuA; Locus:LMHCC_0573
Protein Name2-isopropylmalate synthase
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVKAI AKAIKHCSVTGLARCVEGDIDRAEEALKDAVSPQIHIFLATSDVHMEYKLKMSRAEVLAS IKHHISYARQKFEVVQFSPEDATRSDRAFLIEAVQTAIDAGATVINIPDTVGYTNPTEFG QLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQ DGVLKNPDTYEIITPALVGVDKNSLPLGKLSGKHAFQTRMEEMGYNLSEQELKDAFKRFK QLADAKKEVTEDDLHALILGQSSESADDFELKHLQVQYVTGGVQGAIVRIAERDGTLIED AATGSGSIEAIYNTINRLMKQNIELTNYHIQAITAGQDAQAEVHVVIKDKSGAEFHGIGI DFDVLTASAKAYLQASAKSKTGSKQADFEEVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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