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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP459509BXSYeast1mgQuote
EP459509BXSE. coli1mgQuote
BP459509BXSBaculovirus200ugQuote
MP459509BXSMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315) )
UniProt IDB4E5N2
Gene NameleuA; Locus:BceJ2315_23140; ORFs:BCAL2354
Protein Name
Region Expressed1-514
Expression Tag6xHis
Purity>90%
AA SequenceMTDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKNLERMKVDVIEAGFAASSNGDFDAIHT IAGLVKDSTICSLARANDKDIQRAADALKPANSFRIHTFIATSPLHMEKKLRMTPDQVFE QARLAVRFARKFTDNVEFSPEDGSRSDMDFLCRVLEAVIAEGATTINIADTVGYGVPELY GNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNT SLEEIVMAVKTRKDYFGLDVGIDTSQIVPTSKLVSQITGFVVQPNKAVVGANAFAHASGI HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVSLDSEAELNAAFM RFKDLADRKAEIFDEDIIAIVSEESALAQEQEHFKFVSLSQRSETGEQPQAKVVFAVEGK EVTGEARGNGPVDATFNAIEGEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNG VGTDPDIVAASAKAYISALNKLHSKDDKVNPQRS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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