Request QuoteCatalog Number: xP462046KBOSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP462046KBOYeast1mgQuote
EP462046KBOE. coli1mgQuote
BP462046KBOBaculovirus200ugQuote
MP462046KBOMammalian Cell200ugQuote

Protein Information

SpeciesKocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201)
UniProt IDB2GHT7
Gene NameleuA; Locus:KRH_12930
Protein Name
Region Expressed1-584
Expression Tag6xHis
Purity>90%
AA SequenceMKNLQRPSGMPAHKYTPYHQNFPFDMSDRTWPDKVATTAPRWCAVDLRDGNQALIDPMDS ERKLRMFQLLVGMGYKEIEVGFPSASQTDFDFVRTLVEGGHIPEDVSIQVLTQSREHLIE RTYEAIDGAPNAIVHLYNSTSTLQRRVVFHQDMDGIVDIALTGARLCRKYEEQLSGTAVT YEYSPESYTGTELEFAARICNEVAETFEIGNGRKMIVNLPATVEMATPNVYADSIEWMGR HLYQREDIILSLHPHNDRGTGVAAAELGYLAGADRIEGCLFGNGERTGNVDLVTLGLNLL TQGVDPQIDFSDIEEIRRTVEYCNQLPVPERSPYGGDLVFTAFSGSHQDAIKKGFEAMER DAEQQGVGVDELEWAVPYLPIDPKDVGRSYEAVIRVNSQSGKGGVAYLLKSEYGIDLPRR AQIEFSGVVQKFTETSGSEVSAQQLWTIFRDEYLPSRDGSGSTWGHYRITSISTHAEDQA NTVLEIGLDVGGEHKERTAQGTGPIDALINLFNREGMDVRLLDYTEHTLSASANAQAASY VELAVGDRVLWGAGMDSNTTRASLKAVISAVNRAVRDAQEAAQD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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