Request QuoteCatalog Number: xP463465EYZSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP463465EYZYeast1mgQuote
EP463465EYZE. coli1mgQuote
BP463465EYZBaculovirus200ugQuote
MP463465EYZMammalian Cell200ugQuote

Protein Information

SpeciesProteus mirabilis (strain HI4320)
UniProt IDB4F194
Gene NameleuA; Locus:PMI2084
Protein Name
Region Expressed1-519
Expression Tag6xHis
Purity>90%
AA SequenceMSNNVIIFDTTLRDGEQALQASLSVKEKLQIAYALERLGVDIIEAGFPVSSPGDFESVQT IAREIKNSRICALARCVDNDIDVAAESLNIAEAFRIHVFLATSALHAEHKLKKSFDDIIE MGTRSIKRARRYTDDVEFSCEDAGRTHIDNLCRIVESAINAGATTINIPDTVGYTTPYQF GGIITNLFERVPNIDKAVISVHCHDDLGMAVANSITAVQAGARQVEGTINGLGERAGNCA LEEVIMAIKVREQMMNVQTRINHKEIYRTSQLVSQLCNTPIHANKSIVGSNAFAHSSGIH QDGVLKNRETYEIMTPESIGLKEVQLNLTSRSGRAAVKHRMEEMGYRETDYNLDSLYAAF LRLADKKGQVFDYDLEALAFMGQQQQEPDEFVMNYFNTQSGSSTVATASVSISRKNEEIT EAATGNGPVDAVYQAISRATGYPLKLVTYQLTAKGEGRDALGQVDIVVEYQGRKFHGMGL ETDIVGSSANAMMHVINSIWRSEQVEIEKRKHHTTQEAV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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