Request QuoteCatalog Number: xP531201MTQSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP531201MTQYeast1mgQuote
EP531201MTQE. coli1mgQuote
BP531201MTQBaculovirus200ugQuote
MP531201MTQMammalian Cell200ugQuote

Protein Information

SpeciesMicrocystis aeruginosa (strain NIES-843)
UniProt IDB0JGK2
Gene NameleuA; Locus:MAE_54460
Protein Name2-isopropylmalate synthase
Region Expressed1-533
Expression Tag6xHis
Purity>90%
AA SequenceMNTSPDRVIIFDTTLRDGEQSPGAALNVDEKLTIARALARLGVDVIEAGFPHASPGDFDA VQKIAASVGSEADSPIICGLARTTQKDIKSAADALRPAAKPRIHTFLATSDIHLQYKLKK TRQEVLEIVPEMVAYAKSFVDDVEFSPEDAGRSDPDFLYQVLERAIAAGATTVNIPDTVG YTTPSEFGALIRGIKENVPNIDQAIISVHGHDDLGLAVANFLEAVKNGARQLECTINGIG ERAGNASLEELVMALHVRRSYFNPFLGRPAESTEPLTKINTKEIYRTSRLVSNLTGMIVQ PNKAIVGANAFAHESGIHQDGVLKHKLTYEIMDAESIGLTHNQIVLGKLSGRNAFRSRLQ ELGFELSETELNNAFIQFKEMADRKKEITDRDLEAIVNDEIDTVPDHFRLELVQVSCGNS ARPTATVTIRTPDGSELSDAAIGTGPVDALCKAIDRVVKIPNELISFSVREVTEGIDALG EVTIRLRYEGRTYSAHAADTDIIVASARAYVSALNRLHVALQQKEKTPEMLQV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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