Request QuoteCatalog Number: xP532454HVDSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP532454HVDYeast1mgQuote
EP532454HVDE. coli1mgQuote
BP532454HVDBaculovirus200ugQuote
MP532454HVDMammalian Cell200ugQuote

Protein Information

SpeciesHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
UniProt IDB0TCR1
Gene NameleuA; Locus:Helmi_14620; ORFs:HM1_1515
Protein Name2-isopropylmalate synthase
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMSKRVVIFDTTLRDGEQSPGVSLNLHEKLEIAQQLARLGVDVIEAGFPIASPGDFEAVKA VAEQVRGPVICALARANRKDIERAAEALRGAEEARIHTFIATSPIHMQHKLRMEPDKVLD TAVDAVKLAKSFTSNVEFSAEDAFRSDVGFLCRIFSAAIEAGATTINIPDTVGYATPQEF GAFIKAIINGTPNIDKAIVSVHCHNDLGLAVANTLAALENGALQVEGTINGIGERAGNAS LEEVIMALYTRKPFYNLETSINKSEIYRTSRLVSNLTGMLVQPNKAIVGKNAFAHESGIH QDGVLKERTTYEIMNPEMIGIFTNNIVLGKHSGRHAFRERLKELGYSLDDEKLTKAFARF KALADRKREITDEDLVVLVEDELRAFPEAYSLEYLHITSGTVLVPTATVRLRREEENFEE ASCGDGPVDAAYKAIEKITGTGARLASYAISATTAGEDSQGEVSVKLEREGRFYTGRGVD TDIIVASAKAYLNAVNKIVFDGLPKAKTERAV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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