Request QuoteCatalog Number: xP429314STNSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP429314STNYeast1mgQuote
EP429314STNE. coli1mgQuote
BP429314STNBaculovirus200ugQuote
MP429314STNMammalian Cell200ugQuote

Protein Information

SpeciesShewanella baltica (strain OS195)
UniProt IDA9KY13
Gene NameleuA; Locus:Sbal195_0397
Protein Name2-isopropylmalate synthase
Region Expressed1-522
Expression Tag6xHis
Purity>90%
AA SequenceMSNRVIIFDTTLRDGEQALAASLSVKEKLQIAMALERLGVDVMEVGFPVSSPGDFESVQT IARTIKNSRVCALSRALEKDIDAAAQALSVADQFRIHTFISTSTIHVESKLKRSFDQVLE MAVSAVKYARRFTDDVEFSCEDAGRTPIENLCRMVEAAILAGARTINIPDTVGYTVPSEF GNIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTINGIGERAGNCS LEEIAMILATRKGMLGLETGINAKEIHRTSNLVSQLCNMPVQANKAIVGANAFTHSSGIH QDGMLKAQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMEEMGYSEHDYNMDALYEEF LKLADKKGQVFDYDLEALAFMEAQAEEDNHYQLQQLVVQSDSTEGVATATVRIEVGGEIK TEAATGNGPVDAAYNAIARATDRRIDIISYKLGAKGVGQNALGQVDITAVYHEQNFHGVG LATDVVEASARALVHVMNLTCRADKVADYKQSMQKNRELGGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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