Request QuoteCatalog Number: xP539131UBJSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP539131UBJYeast1mgQuote
EP539131UBJE. coli1mgQuote
BP539131UBJBaculovirus200ugQuote
MP539131UBJMammalian Cell200ugQuote

Protein Information

SpeciesUncultured termite group 1 bacterium phylotype Rs-D17
UniProt IDB1H0A7
Gene NameleuA; Locus:TGRD_456
Protein Name2-isopropylmalate synthase
Region Expressed1-516
Expression Tag6xHis
Purity>90%
AA SequenceMEKDIIIFFDTTLRDGEQSPGASMNLKEKLLVANQLAALGVDVIEAGFPISSQGDFEAVK TISQQIKTSSIAGLCRASEKDITTCWNAVKYAKKPRIHIFLATSDLHIEKKLQKTRAQIL DMAVKTIKYGKSLCKDVEFSAEDAGRSDMDFLCKVVEAVIDAGATTVNIPDTVGYTTPIE FGNKIAEIKRRVPNVNKAIISVHCHNDLGLAVANSLSAIENGARQIECTINGIGERAGNT SLEEIAMTLKVRHHYYNVKHTLKTNELYNTSKLVSRLTGILVQVNKAVVGANAFAHESGI HQDGVLKARETYEIMSPADVGVPESLLVLGKHSGRHAFFKRIKDLGYKLDEKTLEHLFEK FKILADKKKTVFDDDIIALIEEDTSSGKETFILNYLSATSGTGTIPTATVKISKSEGNKK TKSITLQAAACGSGPVDATYKAIDKIINMDIKLTDFSLRSVSSGEDALGEVVLKAEYKGT IYSGKGTSTDIIEASAKAYIQAINKAKLFYDNKKGK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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