Request QuoteCatalog Number: xP544210YAXSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP544210YAXYeast1mgQuote
EP544210YAXE. coli1mgQuote
BP544210YAXBaculovirus200ugQuote
MP544210YAXMammalian Cell200ugQuote

Protein Information

SpeciesYersinia pseudotuberculosis serotype O:3 (strain YPIII)
UniProt IDB1JK98
Gene NameleuA; Locus:YPK_3535
Protein Name2-isopropylmalate synthase
Region Expressed1-520
Expression Tag6xHis
Purity>90%
AA SequenceMSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDIMEVGFPVSSPGDFESVRT IAQQVKNSRVCALARCVDKDIDVAAEALRIAEAFRIHVFLATSTLHIESKLKRSFDDVLA MAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAITAGATTINIPDTVGYTTPYQF GGIITDLYERVPNIDKAIISVHCHDDLGMSVANSITAVQAGARQVEGTINGLGERAGNCS LEEVIMAIKVRHEMLGVHTNINHQEIYRTSQLVSKICNMPIPGNKAIVGSNAFAHSSGIH QDGVLKNRENYEIMTPESIGLKEVQLNLTSRSGRAAVKHRMEEMGYQDKDYNLDSLYDAF LKLADKKGQVFDYDLEALAFINKQQEEPEYYRLDYFSVQSGSSVMATASVKLVCGEEIKS EAATGNGPVDAVYQAINRITDYPIELVKYQLSAKGQGKDALGQVDIVVDHKGRRFHGVGL ATDIVESSAKALVHVLNNIWRAHQVEKEKQRLQQNNQEMV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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