Request QuoteCatalog Number: xP391787AUISize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP391787AUIYeast1mgQuote
EP391787AUIE. coli1mgQuote
BP391787AUIBaculovirus200ugQuote
MP391787AUIMammalian Cell200ugQuote

Protein Information

SpeciesAeromonas salmonicida (strain A449)
UniProt IDA4SR62
Gene NameleuA; Locus:ASA_3409
Protein Name2-isopropylmalate synthase
Region Expressed1-524
Expression Tag6xHis
Purity>90%
AA SequenceMSDRVIIFDTTLRDGEQALAASLTVKEKLQIAQALERLGVDIMEVGFPVSSPGDFQSVQT IARHIKNSRVCALARALPQDIDAAGEALRVAEAFRIHTFISTSSIHVESKLKKSFEDVLE MGVSAIKHALRYTDDVEFSCEDAGRTPIDNLCRMVEAAIKAGARTINIPDTVGYTVPTEF SGIIQTLFNRVPNIDKAIISVHCHDDLGLSVANSIGAVQMGARQIECTINGIGERAGNCS LEEVAMILKTRADLLGVHTNIRHSEIHRTSALVSQLCNMPVQPNKAIVGANAFSHSSGIH QDGVLKAKNTYEIITPESIGLPQNNLNMTSRSGRHVIKHRMESMGYAESSYDLDHLYGKF LTLADKKGQVFDYDLEALAFFSQIHEEPEHFKLEYLGVQSGSSVLATASVKLKVGQDLVC EAATGNGPVDAVYQCINRITGYEIRIDKYALKAKGEGKNALGQVDIVAEYKGRKFHGMGL ATDIIESSAQALIHVINSIWRADQVAEQMERNVTKTDKINTESV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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