Request QuoteCatalog Number: xP392162DHXSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP392162DHXYeast1mgQuote
EP392162DHXE. coli1mgQuote
BP392162DHXBaculovirus200ugQuote
MP392162DHXMammalian Cell200ugQuote

Protein Information

SpeciesDesulfotomaculum reducens (strain MI-1)
UniProt IDA4J181
Gene NameleuA; Locus:Dred_0285
Protein Name2-isopropylmalate synthase
Region Expressed1-505
Expression Tag6xHis
Purity>90%
AA SequenceMNNRVHIFDTTLRDGEQSPGVSLNLNEKLQIARQLARLGVDIIEAGFPITSPGDFAAVQA VAREVRGVTVAGLARANFKDIDVAWDAIKEAEQARIHTFIATSDIHLKYKLRKDREQVIE AAVAAVKHARKYTSDVEFSAEDASRSDIDYLCRIFQEVIKAGATVINVPDTVGYTTPGEY AQFIRDILEKTPGMEKIVLSVHCHDDLGLAVANSLAAVGAGARQVEGAINGIGERAGNAA LEEVVMGLYTRKDYYGLMTGINTPEIYRTSRLVSSLTGMPVQPNKSVVGKNAFAHESGIH QDGVLKERTTYEIMNPEQVGITANNLVLGKHSGRHAFRDRLEELGYHLNEEELNLAFSRF KVLADKKKEITDHDLQAMVEDEIKHIPDTYVLKYLHISTGTAVVPTATLGLQMGDELKEE AACGDGPVDAIYKTVDKITGISCTLVNYAINAITGGKDALGDVTVKIRCEEKEKVYIGRG VSTDILEASAKAYVNAINKLIHDMQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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