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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP391122GEHYeast1mgQuote
EP391122GEHE. coli1mgQuote
BP391122GEHBaculovirus200ugQuote
MP391122GEHMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus thermodenitrificans (strain NG80-2)
UniProt IDA4IRH8
Gene NameleuA; Locus:GTNG_2587
Protein Name2-isopropylmalate synthase
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMRKIKFFDTTLRDGEQSAGVNLNLQEKLEIARQLERLQVDIIEAGFPASSKGDFEAVKQI AETVRTCSVTGLSRSVRSDIDAAWEALKGGAEPRLHLFIATSPIHMVHKLRMTPEQVIEA AVEAVKYAKRFFPIVQWSAEDACRSELPFLAKIVTEVIKAGASVINIPDTVGYITPKEYG EIFLYLRNNVPNIENISLSAHCHDDLGMAVINSLSAIEHGATQVECTINGIGERAGNAAL EEIAVALHIRKDYYQVETRLNLQEIKRTSNLVSKLTGMVVPPNKAVVGKNAFAHESGIHQ DGVLKEKTTYEIISPELVGVSSNSMVLGKHSGRHALRNRVEELGYTLSEEEINQLFVRFK ELADKKKDVTDDDLIALIFEEKFDHFKDFYQLSSIQVQYGTNQIPTAVVVLKDGKGNTIQ EAATGAGSVEALYNTLERCFQTAVTLLDYRIESVGGGRDALAQVFVKVRVNDVETSGRGT AQDVLEASAKAYINAMNRMFMIEAMRAENEKVTTP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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