Request QuoteCatalog Number: xP402378TKFSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP402378TKFYeast1mgQuote
EP402378TKFE. coli1mgQuote
BP402378TKFBaculovirus200ugQuote
MP402378TKFMammalian Cell200ugQuote

Protein Information

SpeciesThermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
UniProt IDA5IJM2
Gene NameleuA; Locus:Tpet_0367
Protein Name2-isopropylmalate synthase
Region Expressed1-513
Expression Tag6xHis
Purity>90%
AA SequenceMRRIKIFDTTLRDGEQSPGASMSVEEKVEMALMLEDLGVDLIEAGFPVSSPVQFEAVKRV ASAVQKPIVVGLARCVEKDIDAVYEALKDRPKDKRMIHVFIATSPIHRKYKLRMEKEEIL ERIRRYVGYAKQFFDLVEFSAEDASRTEVPFLIEAYKTAIEAGATTINVPDTVGYALPDE FGELIKTLREGVPGIENVDLSVHCHNDLGLAVANSLAAVQNGATQVEVTLNGIGERAGNC ALEEFVMILKVRKDKLPYETGIKTELIYPASRLLTHITGLIPSRNKPIVGENVFLHESGI HQDGVLKHRETYEIMKPSDIGRSSETLVLGRHSGKHALRKKLESYGIKLDEETFQKVFEK FTELADRKKEVYDDDLFSIVSEVLREPINGYKLVHFHVHTGNTLLPTAAVVLQVGDEKKE AAEAGNGPVDAIFKAIDKALGIQPKLEEYIIQAVGTGKNAQGEVKLTLRINGELYSGRGV STDIVEASAIAYINAINKYLIAKGLLRKNGGAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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