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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP404859HSDYeast1mgQuote
EP404859HSDE. coli1mgQuote
BP404859HSDBaculovirus200ugQuote
MP404859HSDMammalian Cell200ugQuote

Protein Information

SpeciesHaemophilus influenzae (strain PittEE)
UniProt IDA5UD85
Gene NameleuA; Locus:CGSHiEE_07035
Protein Name2-isopropylmalate synthase
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMTDRVIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSQGDFESVQT IARHIKNARVTALSRAVDKDIDAAYEALKVAEAFRIHTFIASSALHVEAKLKRSFDDVVG MAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINAGATTVNIPDTVGFCLPNEY GNIIAQVRNRVPNIDKAVISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTS LEEVVMAMKVRQDFMGVDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIH QDGMLKNKNTYEIMSPETIGLKKEKLNLTARSGRAAVKGHMADMGYNEQDYDLDKLYDAF LKLADKKGQVFDYDLEALAFIDMQQGDEDRLVLDKLSAHSTKEYPATAFVQLKLDGNKLI TSSIGGNGPVDAVYNAILNLTGLEIKMSHYNLTAKGEGAEALGQVDIVVEHKGRKFHGVG LATDIVESSALALVHAINAIYRAHKVADIKNHKHH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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