Request QuoteCatalog Number: xP402256SVCSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP402256SVCYeast1mgQuote
EP402256SVCE. coli1mgQuote
BP402256SVCBaculovirus200ugQuote
MP402256SVCMammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain WH7803)
UniProt IDA5GM47
Gene NameleuA; Locus:SynWH7803_1586
Protein Name2-isopropylmalate synthase
Region Expressed1-540
Expression Tag6xHis
Purity>90%
AA SequenceMAHDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASPGDFNA VQRIAQQVGGEQGPIICGLARASRGDIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKS RQEVLAIVPEMVAYARSLVDDVEFSCEDAGRSDPEFLYAVIEAAINAGASTINIPDTVGY TTPAEFGALIAGIDQHVPNISEAVLSVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE RAGNAALEELVMALHVRRRYFNPFFGRAEDSPTPLTGVRTEEITKTSRLVSNLTGMVVQP NKAIVGANAFAHESGIHQDGVLKNRLTYEIVDARTVGLTDNRISLGKLSGRSAVRARLEE LGYDLSREDLDDAFARFKELADRKRDITDRDLEAIVSEQVQQPDARYQLKLVQVSCGSSL QPTATVTLLDEEGQEQSEAAIGTGPVDAVCRALNALAGEPNELVEFSVKSVTEGIDAMGE VTIRLRRDGQLFSGHAADTDVVVAAAQAFVNALNRLVAGSLNPTLHPQRDATPLDASPTL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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